The MammaPrint BluePrint Kit consists of four boxes that include the different reagents required for library preparation and target enrichment of up to 16 samples prior to sequencing. Each box has storage temperature requirements specific to its contents.
MammaPrint BluePrint NGS RNA Library Prep (Pre-PCR)Storage: -20°C Box 1 of 4 |
Agendia NGS Fragmentation Mix |
Agendia NGS 1st Strand Master Mix |
Agendia NGS 2nd Strand + End Repair Enzyme Mix |
Agendia NGS 2nd Strand + End Repair Oligo Mix |
Agendia NGS dA Tailing Master Mix |
Agendia NGS Oligo Adaptor Mix |
Agendia NGS Ligation Master Mix |
Agendia NGS Forward PCR Primer |
Agendia NGS PCR Master Mix |
Agendia NGS Uracil DNA Glycosylase (UDG) |
Agendia NGS Reverse PCR Primer |
Agendia NGS Nuclease-Free Water |
MammaPrint BluePrint NGS Target Enrichment (Post-PCR Box 1)Storage: Room Temp Box 2 of 4 |
Agendia NGS Hyb 1 |
Agendia NGS Hyb 2 |
Agendia NGS Hyb 4 |
Agendia NGS Binding Buffer |
Agendia NGS Wash Buffer 1 |
Agendia NGS Wash Buffer 2 |
Agendia NGS Nuclease-Free Water |
Agendia NGS Elution Buffer |
Agendia NGS Neutralization Buffer |
MammaPrint BluePrint NGS Target Enrichment (Post-PCR Box 2)Storage: -20°C Box 3 of 4 |
Agendia NGS Indexing Block 1 |
Agendia NGS Block 2 |
Agendia NGS ILMN Indexing Block 3 |
Agendia NGS RNase Block |
Agendia NGS Hyb 3 |
Agendia NGS Post-Capture PCR Primer |
Agendia NGS PCR Master Mix |
Agendia NGS 8bp Index Plate |
MammaPrint BluePrint NGS Panel
Storage: -80°CBox 4 of 4 |
MammaPrint BluePrint Baits Library |
For a list of equipment and reagents required but not provided with the Kit, please consult the Package Insert, found on the Resources page.